General description
Purified mouse monoclonal antibody. Recognizes the ~19 kDa human α-interferon (KA ≈109 M-1).
Recognizes the ~19 kDa IFN-α protein.
This Anti-α-Interferon Mouse mAb (MMHA-2) is validated for use in ELISA, Immunoblotting, IHC, IP, Neutralization Studies for the detection of α-Interferon.
Immunogen
α-interferon from human leukocytes
Application
ELISA (see comments)
Immunoblotting (1:500)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 18 mM Citric Acid, 165 mM Sodium Phosphate Dibasic.
Other Notes
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.
Rubinstein, S., et al. 1981. J. Virol. 37, 755.
Suitable for neutralization of human α-interferon. Does not recognize β-interferon or γ-interferon by immunoblotting. Effectively neutralizes human α-interferon. This antibody has also been reported to work with ELISA, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | MMHA-2, monoclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (ELISA Immunoblotting (1:500) Immunohistochemistry Immunoprecipitation Neutralization Studies ) |
| isotype | IgG1 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... IFNA1(3439) |
