General description
22 kDa
Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
Immunogen
Epitope: Ser65
Application
Anti-Phospho-Bid (Ser65) Antibody detects level of Phospho-Bid (Ser65) & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Biochem/physiol Actions
Other species have not been tested.
The antibody reacts with the 22 kDa phospho-Bid-S65 protein.
Physical form
Purified in PBS with 0.09% NaN3.
Antigen Affinity Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Western Blot:: Jurkat cell lysate
Evaluated by Western Blot on Jurkat cell lysate treated with (+/-) insulin.
Western Blot Analysis:
A 1:100~500 dilution of this antibody detected phospho-Bid-S65 on jurkat cell lysate treated with (+/-) insulin.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_001196.2 |
| UniProt accession no. | P55957-2 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer65) |
| Gene Information | human ... BID(637) |
