Immunogen
Purified uboquitin covalently coupled to KLH using glutaraldehde.
Application
Anti-Ubiquitin Antibody detects level of Ubiquitin & has been published & validated for use in WB, IH, IH(P).
Immunohistochemistry: 1:1,000-1:5,000. Tissue fixed in 10% formalin, Bouin′s fixative for 1-2 days and embedded in paraffin, or unfixed frozen tissues may be used.
Immunoblotting: 1:1,000-1:5,000.
Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Biochem/physiol Actions
Ubiquitin (76 amino acid, 8.5 kDa). Shows staining of neurofibrillary tangles, neurites in senile plaques and a subset of neuronal processes. No reactivity seen to tau, neurofilament or microtubule-associated proteins.
Physical form
Neat rabbit antisera with 10 mM sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | bovine, eukaryotes, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| NCBI accession no. | NM_021009.4 |
| UniProt accession no. | P62988 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UBAP2(55833) |
