General description
The group of Neuregulin-1 proteins are cell-cell signaling molecules and are ligands for receptor tyrosine kinases of the ErbB/HER subfamily. Type III NRG1 proteins (also referred to as CRD-NRG1 proteins) play a critical role in neural development, including myelination, motor and sensory neuron survival, and neuromuscular synapse development (Falls, 2003). Defects in animals lacking type III NRGs include retraction of nerve terminals from newly formed synapses, absence of Schwann cells from peripheral nerves, and loss of motor and sensory neurons (Wolpowitz et al., 2000). Available evidence indicates that most Type III NRG1 proteins are synthesized as transmembrane proteins with 2 TM domains, and that proteolytic processing produces a bioactive transmembrane form (NTFm) which serves as a juxtacrine signal (Wang, 2001; Falls, 2003).
~29 kDa observed. This target has been shown to undergo differential splicing during development into ~30 different isoforms.
Immunogen
Epitope: Extracellular domain
His-tagged recombinant protein corresponding to mouse Neuregulin-1 at the extracellular domain.
Application
Detect Neuregulin-1 using this Anti-Neuregulin-1 Antibody, clone D11 validated for use in WB & IC.
Immunocytochemistry Analysis: A previous lot of this antibody was used successfully by an independent laboratory in IC. (Pirotte, D., et al.)
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Biochem/physiol Actions
This antibody recognizes Neuregulin-1 at the extracellular domain.
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain microsomal tissue lysate
Evaluated by Western Blot in mouse brain microsomal tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Neuregulin-1 on 10 µg of mouse brain microsomal tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | D11, monoclonal |
| species reactivity | mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_848706 |
| UniProt accession no. | Q6DR99 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Nrg1(211323) |
