General description
A specific and reversible inhibitor of CPP32/Apopain/Yama (IC50 = 0.2 nM). A member of the ICE/CED-3 family of cysteine proteases, which are important in apoptosis.
A very potent, specific, and reversible inhibitor of caspase-3 (IC50 = 200 pM), caspase-6, caspase-7, caspase-8, and caspase-10.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Capase-3, capase-6, capase-7, capase-8, capase-10
Product does not compete with ATP.
Reversible: yes
Target IC50: 200 pM against caspase-3
Packaging
Yes
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Ac-Asp-Glu-Val-Asp-CHO
Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W. 1996. Nature Biotech. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Nicholson, D.W., et al. 1995. Nature376, 37.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| Quality Level | 100 |
| assay | ≥90% (HPLC) |
| form | solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| color | white |
| solubility | DMSO: 5 mg/mL,water: soluble |
| shipped in | ambient |
| storage temp. | −20°C |
| SMILES string | N([C@@H](C(C)C)C(=O)N[C@@H](CC(=O)O)C=O)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(=O)O)CCC(=O)O |
| InChI | 1S/C20H30N4O11/c1-9(2)17(20(35)22-11(8-25)6-15(29)30)24-18(33)12(4-5-14(27)28)23-19(34)13(7-16(31)32)21-10(3)26/h8-9,11-13,17H,4-7H2,1-3H3,(H,21,26)(H,22,35)(H,23,34)(H,24,33)(H,27,28)(H,29,30)(H,31,32)/t11-,12-,13-,17-/m0/s1 |
| InChI key | UMBVAPCONCILTL-MRHIQRDNSA-N |
