General description
84 kDa calculated
Prostate-specific membrane antigen (PSMA), also known as Glutamate carboxypeptidase II (GCPII), Folate hydrolase 1 (FOLH1), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I), is a member of the peptidase M28 family and is involved in prostate tumor progression. It is highly expressed in prostate epithelium and detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, and brain (at protein level). Mutations in PSMA have been associated with prostate cancer and hyperhomocysteinemia. PSMA may be a possible marker for neurological disorders such as schizophrenia, Alzheimer′s disease, and Huntington disease.
Immunogen
Recombinant protein corresponding to human PSMA.
Application
Anti-PSMA Antibody, clone 3/A12 is a highly specific mouse monoclonal antibody, that targets PSMA & has been tested in Flow Cytometry, western blotting, Immunofluorescence, Activity Assay & Inhibition.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: A representative lot from an independent laboratory detected purified PSMA in WB (Elsasser-Beile, U., et al. (2006). Prostate. 66(13):1359-1370.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected PSMA in LNCaP cells (Elsasser-Beile, U., et al. (2006). Prostate. 66(13):1359-1370.).
Inhibition Assay Analysis (Small-animal PET imaging): A representative lot from an independent laboratory blocked PSMA in mice bearing PSMA-positive C4-2 tumors and negative DU145 tumors (Elsasser-Beile, U., et al. (2009). J Nucl Med. 50(4):606-611.).
Activity Assay Analysis: A representative lot from an independent laboratory was co-internalized with cell adherent PSMA, when PSMA binds to its putative ligand (Liu, J. et al. (2009). Mol Pharm. 6(3):959-970.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Flow Cytometry in 1X10E6 LNCaP cells.
Flow Cytometry Analysis: 1 µg of this antibody detected PSMA in LNCaP cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABN1035
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3/A12, monoclonal |
| species reactivity | human |
| technique(s) | activity assay: suitable,flow cytometry: suitable,immunofluorescence: suitable,inhibition assay: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001014986 |
| UniProt accession no. | Q04609 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FOLH1(2346) |
