产品介绍
Product Description
General description
Heterobifunctional reagent useful for preparation of conjugates such as secondary antibodies and β-galactosidase for EIA procedures or for immobilizing enzymes on solid supports.
N-γ-maleimidobutyryloxysuccinimide ester (GMBS) is an aliphatic crosslinker for immunotoxins (IT).(1) It is used for the generation of conjugated Poly-γ-D-glutamic acid.
Application
GMBS is suitable for use:
- as a hetero-bifunctional cross-linking reagent for the immobilization of neural adhesion molecule L1 onto silicon dioxide surface
- for the crosslinking of biochips with functionalized gold nanoparticles
- for coating salinized capillary tube prior to neutravidin treatment in biosensor sandwich immunoassay
Preparation Note
Reconstitute to 10 mg/ml in tetrahydrofuran or DMSO. Add 1 ml of this stock solution dropwise, with stirring, to 10 ml of 20 mM phosphate buffer.
Other Notes
Rusin, K.M., et al. 1992. Biosens. Bioelectron. 7, 367.
Kitagawa, T., et al. 1983. J. Biochem. 94, 1160.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
技术参数
Specifications
| assay | ≥90% (HPLC) |
| Quality Level | 100 |
| form | solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| storage temp. | −20°C |
| SMILES string | N2(C(=O)CCC2=O)OC(=O)CCCN1C(=O)C=CC1=O |
| InChI | 1S/C12H12N2O6/c15-8-3-4-9(16)13(8)7-1-2-12(19)20-14-10(17)5-6-11(14)18/h3-4H,1-2,5-7H2 |
| InChI key | PVGATNRYUYNBHO-UHFFFAOYSA-N |
