Application
Immunohistochemistry: 1:500-1:2,000 on paraffin embedded sections (ABC peroxidase) fixed with alcohol (e.g. Carnoy) with a 2" pronase digestion. Aldehyde fixation is not recommended.
Immunocytochemistry: 1:100-1:200 cells in vitro (IFA) fixed in ice cold absolute methanol or acetone.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Vimentin Antibody, clone 43BE8 is validated for use in IC, IH(P) for the detection of Vimentin.
Biochem/physiol Actions
Vimentin (58kD). Reacts in immunocytochemical assays with all mesenchymal cells (fibroblasts, Scwann cells, endothelial cells, smooth muscle cells, etc.).
Physical form
Liquid
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 43BE8, monoclonal |
| species reactivity | human, monkey |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin) |
| isotype | IgM |
| NCBI accession no. | NM_003380.2 |
| UniProt accession no. | P08670 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... VIM(7431) |
