General description
Mouse monoclonal antibody purified by ammonium sulfate precipitation and DEAE column chromatography. Recognizes the ~240 kDa α-subunit of intracellular and plasma membrane-associated fodrin.
Recognizes the ~240 kDa intracellular and plasma membrane associated β subunit of fodrin.
This Anti-Fodrin Mouse mAb (Fod009) is validated for use in ELISA, Flow cytometry, Immunoblotting, Immunoprecipitation for the detection of Fodrin.
Immunogen
Bovine
purified bovine brain fodrin
Application
ELISA (1:1000)
Flow cytometry (1:500)
Immunoblotting (1:200)
Immunohistochemistry (1:100)
Immunoprecipitation (1:100)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Lokeshwar, V.B., and Bourguignon, L.Y.W. 1992. J. Biol. Chem. 267, 22073.
Bourguignon, L.Y., and Bourguignon, G.J. 1984. Int. Rev. Cytol.87, 195.
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | Fod009, monoclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | rat, human, bovine, canine, porcine, rabbit, mouse |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (ELISA (1:1000) Flow cytometry (1:500) Immunoblotting (1:200) Immunohistochemistry (1:100) Immunoprecipitation (1:100)) |
| isotype | IgG2a |
| shipped in | wet ice |
| storage temp. | −70°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SPTAN1(6709) |
