Immunogen
Epitope: phospho-specific (Ser19)
Synthetic phosphopeptide from human myosin, smooth and non-muscle regulatory light chain.
Application
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Myosin Light Chain Antibody, phospho-specific (Ser19) is validated for use in ELISA, WB for the detection of Myosin Light Chain.
Western blot: 1:1,000-1:5,000 using ECL.
ELISA: 1:120,000-1:135,000
Immunohistochemistry: 2.5 μg/mL
Biochem/physiol Actions
Myosin, human, smooth and non-muscle regulatory light chain, phosphoSer19. By Western blot the antibody recognizes the ~20 kDa phosphorylated protein. The immunogen sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain. The antibody recognizes monophosphorylated (pSer19) and diphosporylated (pThr18-pSer19) forms of the protein. Reactivity to non-phosphorylated human myosin, smooth and non-muscle regulartory light chain is minimal (less than 1%) by ELISA.
Physical form
Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.
Preparation Note
Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| NCBI accession no. | NM_002475.3 |
| UniProt accession no. | P14649 |
| shipped in | dry ice |
| target post-translational modification | phosphorylation (pSer19) |
| Gene Information | human ... MYBPHL(343263) |
