General description
Antigen-presenting glycoprotein CD1d (UniProt: P15813; also known as R3G1, CD1d) is encoded by the CD1D gene (Gene ID: 912) in human. Cd1d is a single-pass type 1 membrane glycoprotein, which is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 20-301), a transmembrane domain (aa 302-322), and a short cytoplasmic domain (aa 323-335). It is expressed at high levels by human cortical thymocytes, and its expression is also observed on certain T cell leukemias. It serves as a key antigen-presenting molecule involved in the selection and activation of NK cells. It is a major-histocompatibility-complex (MHC) class-I-like molecule that presents self-lipid and non-self-lipid antigens to invariant natural killer T (iNKT) cells. Following engagement of the invariant T cell receptor (iTCR) by CD1d lipid complexes, iNKT cells proliferate, produce cytokines, and become cytotoxic, regulating innate and adaptive immune responses. (Ref.: Oleinika, K., et al. (2018). Nat. Commun. 9(1); Article 684; Spada, FM., et al. (2000). Eur. J. Immunol. 30; 3468-3477; Exley, M., et al. (2000). Immunology. 100(1): 37-47).
Immunogen
Recombinant fusion protein composed of 1, 2 and 3 domains of human CD1d fused to the Fc portion of murine IgG2a.
Application
Quality Control Testing
Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: 1.0 µg of this antibody detected CD1d in one million Human peripheral blood mononuclear cells (PBMC).
Tested Applications
Inhibition Assay: A representative lot inhibited the responses of NK T cell clones to -GalCer in the presence of monocyte lineage APC. (Spada, F.M., et al. (2000). Eur J Immunol. 30(12):3468-77).
Flow Cytometry Analysis: A representative lot detected CD1d in Flow Cytometry (Spada, F.M., et al. (2000). Eur J Immunol. 30(12):3468-77).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone CD1d42 is a mouse monoclonal antibody that detects CD1d. It targets an epitope within the extracellular domain.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Preparation Note
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | CD1d42, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 100 |
| technique(s) | flow cytometry: suitable,inhibition assay: suitable |
| isotype | IgG1κ |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_001306074 |
| UniProt accession no. | P15813 |
| target post-translational modification | unmodified |
| Gene Information | human ... CD1D(912) |
