General description
Suppressor of variegation 4-20 homolog 2 (Suv4-20h2), also known as Lysine N-methyltransferase 5C (KMT5C), is a Histone-lysine N-methyltransferase that specifically trimethylates Lys-20 of histone H4. Suv4-20h2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. In mammals, Histone H4 Lys-20 trimethylation (H4K20me3) is one of the most common chromatin modifications and it has been implicated in various biological processes, including heterochromatin formation, telomere function, imprinting, X inactivation, DNA damage repair and cancer progression.
~53 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~11 kDa and ~15 kDa
Immunogen
GST-tagged recombinant protein corresponding to human Suv4-20h2.
Application
Detect the Suv4–20h2 protein using this Anti-Suv4–20h2, clone 6B9 validated for use in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
H9 human embryonic stem cell lysate
Evaluated by Western Blot in H9 human embryonic stem cell lysate.
Western Blot Analysis: 4.0 µg/mL of this antibody detected Suv4-20h2 in 10 µg of H9 human embryonic stem cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 6B9, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_116090 |
| UniProt accession no. | Q86Y97 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SUV420H2(84787) |
