General description
68 kDa
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells.
Immunogen
Epitope: Ser126
KLH-conjugagted synthetic peptide corresponding to human Paxillin at phosphorylated Ser126.
Application
Anti-phospho-Paxillin (Ser126) Antibody is an antibody against phospho-Paxillin (Ser126) for use in WB.
Research Category
Signaling
Research Sub Category
G-proteins
Biochem/physiol Actions
This antibody recognizes Paxillin phosphorylated at Ser126.
Physical form
Affinity purified
PBS (no Ca2+, no Mg2+), pH 7.3, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
Preparation Note
Maintain at -20°C for up to one year or 2-8°C for short term use from date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Pervandate treated and untreated Huvec cell lysate
Evaluated by Western Blot in pervandate treated and untreated Huvec cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Paxillin on 10 µg of Pervandate treated and untreated Huvec cell lysate.
Other Notes
Replaces: AB3793
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_002850.2 |
| UniProt accession no. | P49023 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer126) |
| Gene Information | human ... PXN(5829) rat ... Pxn(360820) |
