General description
Immunoaffinty purified rabbit polyclonal antibody. Recognizes the ~180 kDa NMDAR2B subunit of the NMDA receptor.
Recognizes the ~180 kDa NMDA receptor NR2B subunit.
This Anti-NMDAR 2B (984-1104) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of NMDAR 2B (984-1104).
Immunogen
Rat Brain
a recombinant fusion protein consisting of amino acids 984-1104 of rat brain NMDAR 2B-subunit
Application
Immunoblotting (1:1000)
Immunohistochemistry (1:1000-1:2000)
Immunoprecipitation (see comments)
Physical form
Lyophilized from 5 mM ammonium bicarbonate.
Preparation Note
Reconstitute in 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze thaw cycles of reconstituted solution.
Other Notes
Does not cross-react with NMDAR2A or NMDAR2C. Please refer to the certificate of analysis for lot-specific immunoprecipitation volume. Variables associated with assay conditions will dictate the proper working dilution.
Wang, Y.H., et al. 1995. J. Neurochem.65, 176.
Petralia, R.S., et al. 1994. J. Neurosci.14, 6102.
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Nature256, 1217.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | lyophilized |
| does not contain | preservative |
| species reactivity | rat, fish, mouse, human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (Immunoblotting (1:1000) Immunohistochemistry (1:1000-1:2000) Immunoprecipitation ) |
| isotype | IgG |
| shipped in | ambient |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
