Immunogen
Epitope: S. japonicum form
Recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.
Application
Anti-Glutathione-S-Transferase Antibody, S. japonicum form is an antibody against Glutathione-S-Transferase for use in ELISA & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
The anti-GST antibody can be used to detect GST fusion proteins in Western blot analysis and ELISA studies.
Biochem/physiol Actions
Anti-Glutathione S-transferase (GST) is a polyclonal antibody to the recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.
The anti GST antibody reacts specifically with the glutathione S-transferase moiety of GST fusion proteins as expressed in E.coli by the pGEX (1) vectors and in yeast by the pYEX(TM) vectors (2).
(1)The pGEX expression vector system is a plasmid and purification system which, in most cases, results in the fusion protein being soluble in aqueous solutions for extraction and purification from crude bacterial lysates. This expression vector system enables the protein glutathione S-transferase (GST) to be produced in fusion with the protein of interest so as to bind with high affinity to a glutathione affinity column. The GST-protein complex can then be easily purified by elution from the column with reduced glutathione. The pGEX vectors are available as the GST System from Pharmacia Biotech.
(2) pYEX
Physical form
Format: Purified
Liquid in PBS, pH 7.4, containing 0.02% Tween 20 and 0.02% sodium azide as a preservative.
Preparation Note
The anti GST antibody is shipped liquid at 4°C. The anti GST antibody is stable for up to 6 months when stored at 2-8°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | Schistosoma japonicum, vertebrates |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| NCBI accession no. | NM_000561.2,NM_146421.1 |
| UniProt accession no. | P09488 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... GSTP1(2950) |
