General description
Paired mesoderm homeobox protein 2A (PHOX2A), also known as ARIX1 homeodomain protein (ARIX) and Paired-like homeobox 2A (PMX2A), along with the closely related PHOX2B, is a paired-homeodomain transcription factor that maintains the noradrenergic phenotype during embryogenesis. PHOX2 proteins help regulate endogenous hydroxylases in neural crest cells and aid in sympathetic neuron generation. PHOX2A controls only the differentiation of the main noradrenergic center of the human brain, while PHOX2B is required for the differentiation of all central and nonperipheral noradrenergic centers. PHOX2A may be involved in regulating specific expression of catecholamine biosynthetic genes. PHOX2A is linked to congenital fibrosis of extraocular muscles type 2 (CFEOM2). PHOX2 regulation may have therapeutic applications for aging or degenerative noradrenergic neuron disorders.
~32 kDa observed. Uncharacterized bands may appear in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to human PHOX2A.
Application
Research Sub Category
Neurodegenerative Diseases
Anti-PHOX2A Antibody is a highly specific rabbit polyclonal antibody, that targets Phox2a & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PHOX2A in human brain tissue.
Research Category
Neuroscience
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Evaluated by Western Blotting/Peptide Inhibition Assay in rat brain tissue lysate.
Western Blotting/Peptide Inhibition Assay Analysis: 1µg/mL of this antibody detected PHOX2A in 15 µg of rat brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, rat |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| UniProt accession no. | O14813 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PHOX2A(401) |
