Immunogen
Highly pure (>98%) recombinant human SDF-1alpha.
Application
Detect Stromal Cell-Derived Factor-1α using this Anti-Stromal Cell-Derived Factor-1α Antibody validated for use in ELISA, WB, NEUT.
ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of 0.2-0.4 ng/well when human SDF-1alpha is used to coat the plate.
Western blot: 0.1-0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.
Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human SDF-1alpha (100 ng/mL), a concentration of 2.0-0.4 ng/well of recombinant human SDF-1alpha
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Biochem/physiol Actions
Reacts with human SCDF-1alpha
Physical form
Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water.
Preparation Note
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,neutralization: suitable,western blot: suitable |
| NCBI accession no. | NM_000609.4,NM_001033886.1,NM_199168.2 |
| UniProt accession no. | P48061 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CXCL12(6387) |
