Immunogen
Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.
Application
Immunoprecipitation
Immunocytochemistry (acetone fixation required)
Flow cytometry
Inhibits platelet aggregation, 10 μg/mL
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin β3 Antibody, clone 25E11 is validated for use in FC, IP, IC, FUNC for the detection of Integrin β3.
Biochem/physiol Actions
Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. The apparent molecular weight of the GPIIIa by SDS-PAGE is 105 kDa reduced and 90 kDa unreduced. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.
(original paper claimed IgG2ak)
Physical form
Format: Purified
In buffer of 0.02M phosphate, 0.25M sodium chloride, pH 7.6, 0.1% sodium azide.
Preparation Note
Maintain between 2 and 8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1060
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 25E11, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgM |
| NCBI accession no. | NM_000212.2 |
| UniProt accession no. | P05106 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGB3(3690) |
