General description
Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling.
Immunogen
Bone Sialoprotein isolated from human bones.
Epitope: C-terminus
Application
Research Category
Cell Structure
Research Sub Category
Osteobiology
This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II.
Western blot: (1μg/mL)
Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL)
Immunocytochemistry: (1:100)
ELISA: (1μg/mL)
RIA
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin.
Physical form
Format: Purified
Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4.
Preparation Note
Maintain lyophilized material at 2-8°C. Reconstitute with 100μL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB1855
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | ID1.2, monoclonal |
| species reactivity | human |
| should not react with | bovine, rat, pig |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),radioimmunoassay: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_004967.2 |
| UniProt accession no. | P21815 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... IBSP(3381) pig ... Ibsp(397137) rat ... Ibsp(24477) |
