General description
Frizzled 5 (62kDa)
The Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis. The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), β- and γ- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors. Frizzled 5 is believed to function as the receptor for the Wnt5a ligand.
Immunogen
Epitope: C-Terminus
KLH-conjugated, synthetic peptide derived from amino acids 564-585 of human Frizzled 5 (CTGRTPPGPAATYHKQVSLSHV).
Application
Anti-Frizzled 5 Antibody is an antibody against Frizzled 5 for use in WB.
Research Category
Signaling
Research Sub Category
Developmental Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Biochem/physiol Actions
Frizzled 5 (aka Fz5 or Fzd5)
Physical form
Format: Purified
Protein A chromatography
Purified antibody in PBS with 0.05% sodium azide and 30% Glycerol
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Lysates from mouse liver, C2C12, Jurkat, and HUVEC
Routinely evaluated by immunoblot on lysates from mouse liver, C2C12, Jurkat or HUVEC cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | rat, human, mouse |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_003459 |
| UniProt accession no. | Q13467 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FZD5(7855) |
