General description
Placenta-expressed transcript-1 (Plet-1) is a recently identified protein that as of yet has no known function and is not associated with any one family of proteins. Its observation has been restricted to developing mesonephros and pharyngeal endoderm. Observation in adult tissue is more widely expressed, having been found in several locations such as mammary, pancreas and prostate epithelia. Its location in the pancreas is notable as the pancreas is a well known reserve for stem cells and progenitor cells. Within the pancreas, Plet-1 has been shown to be localized in the major duct epithelium, and as such will serve as an excellent marker for epithelial progenitor cells and stem cells, and as a useful implement for observing the genetics of linear relationships and the operation of molecular mechanisms for homeostasis, development and wound healing in organ and tissue systems.
Recently, Plet-1 has been identified as a specific marker of early thymic epithelial progenitor cells. In the postgastrulation embryo, Plet-1 expression is highly restricted to the developing pharyngeal endoderm and mesonephros. This antibody recognizes the same protein as MTS20 and MTS24.
~ 23 kDa
Immunogen
Epitope: Unknown
Full length recombinant protein corresponding to Plet-1.
Application
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Depreter, M.G.L., et al., 2008).
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Depreter, M.G.L., et al., 2008).
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Depreter, M.G.L., et al., 2008).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-Plet-1 (Placenta-expressed transcript 1 protein) Antibody, clone 1D4 is validated for use in WB, IC, IH, FC for the detection of Plet-1 (Placenta-expressed transcript 1 protein).
Biochem/physiol Actions
This antibody recognizes Plet-1.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human placenta cell lysate
Evaluated by Western Blot in human placenta cell lysate.
Western Blot Analysis: A 1:2,500 dilution of this antibody detected Plet-1 in 10 µg of human placenta cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1D4, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology) |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001138496.1 |
| UniProt accession no. | Q6UQ28 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PLET1(349633) |
