Application
Donkey anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Mouse IgG for use in ELISA & WB.
EIA and Western blots: 1:5,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Biochem/physiol Actions
Specific for mouse IgG, heavy and light chain.
Physical form
Immunoaffinity Purified and Immunodepletion
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.15M NaCl, pH 7.1 with 15 mg/mL BSA.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
Preparation Note
Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | donkey |
| Quality Segment | 100 |
| conjugate | peroxidase conjugate |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| shipped in | wet ice |
| target post-translational modification | unmodified |
