General description
Jumonji domain-containing protein 2A (JMJD2A), or KDM4A, is a ubiquitously expressed demethylase enzyme, which belongs to the JMJD2 family. Like other members of this family, JMJD2A contains multiple domains: two Tudor domains, two PHD-type zinc fingers, one JmjC domain, and one JmjN domain. Little is known about the function of most of these domains; however, they are believed to be involved in transcriptional regulation. JMJD2A functions as a histone demethylase with specific activity against trimethylated Histone H3 Lysine residues Lys-9 and Lys-36. It has no effect on any other lysine residues, or on mono- and dimethylated Lys-9 and Lys-36. The demethylation of these residues induces the production of succinate and formaldehyde. Studies have shown that JMJD2A recruits NCOR1 and histone deacetylases, causing the respective transcriptional repression of E2Fand ASCL2 responsive promoters.
~120 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human JMJD2A.
Application
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected JMJD2A in human colorectal adenocarcinoma cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
This Anti-JMJD2A Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of JMJD2A.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
U2OS nuclear extract
Evaluated by Western Blot in U2OS nuclear extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected JMJD2A in 10 µg of U2OS nuclear extract.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 8B7.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_055478 |
| UniProt accession no. | O75164 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... KDM4A(9682) |
