产品详情

RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli,10KU,553504-10KUCN,Sigma

销售价: ¥ 6010.71 / 件
优    惠: 请询价
订货号 0BV5155
品牌型号 553504-10KUCN
货期 询货期
最小订货量 1件
产品介绍 Product Description

General description

Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.


Packaging

Please refer to vial label for lot-specific concentration.


Physical form

In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.


Other Notes

One unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
West, S.C. 2003. Nat. Rev. Mol. Cell Biol.4, 435.
Jasin, M. 2002. Oncogene21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev.66, 630.
Daboussi, F., et al. 2002. Cell. Signal.14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res.4, 9.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
assay≥95% (SDS-PAGE)
Quality Segment100
formliquid
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
shipped inwet ice
storage temp.−70°C
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