General description
Purified mouse monoclonal antibody. Recognizes the ~48-50 kDa TUBB2A protein.
Recognizes the ~48-50 kDa TUBB2A protein in PC-12, Raw 264.7, Jurkat, and NIH3T3 cells and human spleen tissue.
This Anti-TUBB2A Mouse mAb (2B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of TUBB2A.
Immunogen
Full-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
Application
Immunoblotting (1-5 g/ml)
Paraffin Sections (1.5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
PC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
Other Notes
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2B2, monoclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | human, mouse, rat |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Immunoblotting (1-5 µg/mL) Paraffin Sections (1.5 µg/mL)) |
| isotype | IgG2a |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TUBB2A(7280) |
