General description
Galectin 4, or GAL-4, is another member of the Galectin family. Galectins are carbohydrate binding proteins that appear important in cell-cell contact, cell migration, and cell to cell signaling in both normal and disease (cancerous) tissues. Galectin 4 differs from its sister family members in the range of carbohydrate substrates that it binds. Galentin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine. It is often found in relatively insoluble complexes as a component of either adherent junctions or lipid rafts in the microvillus membrane, and it has been proposed to stabilize these structures. Strong expression of galectin-4 can be induced, however, in cancers from other tissues including breast and liver. Also, Galectin-4 appears to specifically bind CD3 on activated T-cells in the gut and regulate their activity via calpain mediated apoptosis.
~36 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: near N-terminal
KLH-conjugated linear peptide corresponding to human Galectin-4 near the N-terminal.
Application
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Galectin-4 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Galectin-4.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of Colo320 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of human small intestine tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Galectin-4 in human stomach tissue.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Galectin-4 in 10 µg of HT-29 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | primate (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), feline (based on 100% sequence homology), monkey (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_006140 |
| UniProt accession no. | P56470 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LGALS4(3960) |
