Immunogen
Purified human erythroid cells ankyrin
Application
Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals.
ELISA: 1:1000
Immunoprecipitation: 1:100
Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10′ RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.
Flow cytometry: 1:500
Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.
RIA: 1:5000
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues).
Physical form
Format: Purified
Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | Ank016, monoclonal |
| species reactivity | rat, human, rabbit, pig, mouse, bovine, canine |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2a |
| NCBI accession no. | NM_000037.3,NM_020475.2,NM_020476.2,NM_020477.2,NM_020478.3,NM_020479.2,NM_020480.3,NM_020481.2 |
| UniProt accession no. | P16157 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ANK1(286) |
