General description
Hyaluronan or hyaluronic acid, also known as Hyaluronan synthase 2 or Hyaluronate synthase 2 or Hyaluronic acid synthase 2 (HA synthase 2), and encoded by the gene name HAS2, is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1.
~55 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human HAS2 expressed in E. Coli
Application
Research Sub Category
Apoptosis - Additional
Anti-HAS2, clone 4E7 Antibody is an antibody against HAS2 for use in western blotting, IHC, immunofluorescence & ELISA.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected HAS2 in ovarian cancer tissue.
Immunofluorescence Analysis: A 1;100-500 dilution from a representative lot detected HAS2 in HeLa cells.
ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Apoptosis & Cancer
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human plasma and Jurkat lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected HAS2 in human plasma and Jurkat lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4E7, monoclonal |
| species reactivity | human |
| technique(s) | ELISA: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | Q92819 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HAS2(3037) |
