General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.
Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
This PhosphoDetect
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
Application
Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)
Physical form
In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Do not aliquot.
Analysis Note
Positive Control
Lysates from COS cells treated with UV
Other Notes
Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | monkey, rat, mouse, human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (Immunoblotting (1:1000) Immunocytochemistry (1:500, fluorescence) Paraffin Sections (1:50)) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer317/pSer317) |
| Gene Information | human ... CHEK1(1111) |
