UCOE® Single Expression Hygromycin Vector Set，1KIT，UC0E02，Sigma

General description

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 HS-hygro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

CET 1019 AS-hygro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

The CET 1019 HS-hygro-SceI and CET 1019 AS-hygro-SceI vectors possess the following features:

• A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
• A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
• A hygromycin antibiotic resistance gene for selection of stably transfected mammalian cells
• A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
• An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes

Application

Research Category
General Cell Biology

All
Research Sub Category
All
Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing.

Preparation Note

Plasmids should be stored at -20C. Avoid repeated freeze-thaw events.

Other Notes

1) 10ug CET 1019 HS-hygro-SceI (+) Vector (CS221288)

2) 10ug CET 1019 AS-hygro-SceI (+) Vector (CS221289)

3) 10ug SC HS-hygro (-) Vector (CS221290)

4) 10ug SC AS-hygro (-) Vector (CS221291)
Concentration: Please refer to lot specific datasheet.

Legal Information

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.