Application
DNA/RNA G-quadruplexes (G4s) are four-stranded nucleic acid structures that fold from numerous guanine (G)-rich sequences found in the genome and transcriptome. Their abundance and localization in critical regulatory regions make them key players in genetic diseases (cancer, neuropathology), but not only (e.g., viral infection). For this reason G4s have garnered intense interest as potential targets in different therapeutic areas.
The BioTracker™ BioCyTASQ G-quadruplex G4 cell probe is optimized for detection (1) and/or the isolation of G4s (2,3). BioCyTASQ utilizes the template-assembled synthetic G-quartets (TASQ) (4) concept as a biomimetic ligand for G-quadruplexes, a design which results in high specificity for folded G4.
References1.ACS Chem Biol. 2021, 16: 905–914 2. Nature Protoc. 2022, 17: 870–889 3. STAR Protoc. 2024, 5: 103480 4. Acc. Chem. Res. 2023, 56: 350–362 5. iScience 2023, 26:106846
Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis
Features and Benefits
BioCyTASQ is suited to visualize G4s in cells in a 2-step protocol referred to as pre-targeted G4 imaging, which relies on the use of a fluorescent streptavidin (1). BioCyTASQ is also suited to isolate G4s by affinity capture experiments, in a protocol named G4RP for RNA G4s, (2,3) or G4DP for DNA G4s (5)). These G4-selective chemoprecipitations allow for their identification by either PCR, RT-PCR, or sequencing.
Physical form
Lyophilized. White solid.
Preparation Note
Store BioTracker BioCyTASQ G-quadruplex G4 cell probe at -20°C, desiccated and protected from light
Other Notes
Fluorescent detection of nucleic acid G4 quadruplexes in cells and tissues
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| packaging | vial of 1 mg |
| Quality Level | 100 |
| manufacturer/tradename | Millipore |
| growth mode | N/A |
| technique(s) | DNA sequencing: suitable,RNA extraction: suitable,nucleic acid detection: suitable |
| detection method | fluorometric |
| shipped in | dry ice |
| storage temp. | −20°C |
