General description
39 kDa
In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi1 is a selective marker for intestinal stem or early lineage cells. Musashi1 interacts with the Notch pathway during asymmetric cell division by binding the 3′ UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi1 is present.
Immunogen
Synthetic peptide, amino acids 5-21 of Musashi.
Application
Detect Musashi-1 using this Anti-Musashi-1 Antibody validated for use in IC, IH & WB.
Immunohistochemistry:
1:200-1:1,000 dilution from a previous lot was used.
Immunocytochemistry:
1:200-1:1,000 dilution from a previous lot was used.
Western blot:
1:200-1:1,000 using ECL.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Developmental Neuroscience
RNA Binding Protein (RBP)
Biochem/physiol Actions
Reactivity with other species has not been confirmed.
Recognizes Musashi-1.
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Preparation Note
Stable for 1 year at -80ºC from date of receipt.
Analysis Note
Control
Mouse cortical neural stem cells (Catalog No. SCR029).
Routinely evaluated by Western Blot on Human Placenta lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Musashi-1 on 10 μg of Human placenta lysates.
Other Notes
Replaces: 03-114; 04-1041
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rodent, mouse, rat, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NM_002442.2 |
| UniProt accession no. | O43347 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MSI1(4440) |
