General description
IgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.
Immunogen
Epitope: IgM µ chain
Prepared from purified mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.
Application
ELISA: 1:5,000-1:20,000
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
This Goat anti-Mouse IgM µ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA for the detection of Mouse IgM µ chain.
Biochem/physiol Actions
Reacts with heavy chain of mouse IgM. Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.
The antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Physical form
Affinity Chromatography
Purified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.
Preparation Note
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Segment | 100 |
| conjugate | biotin conjugate |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| concentration | 0.5 mg/mL |
| technique(s) | ELISA: suitable |
| shipped in | wet ice |
| target post-translational modification | unmodified |
