General description
Uracil-DNA glycosylase 1 (UNG1), also known as UDG1, DGU or UNG15, hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, and as a result it releases free uracil. UNG1 excises uracil residues from the DNA. This event could be a result of faulty incorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Uniport describes two isoforms. The fist isoform is widely expressed with the highest expression in skeletal muscle, heart and testicles. The second isoform has the highest expression levels in tissues containing proliferating cells. Recent studies have shown that defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). HIGM5 is a rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections.
~37 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~34 kDa and ~35 kDa
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human UNG1.
Application
Anti-UNG1 Antibody is a highly specific rabbit polyclonal antibody, that targets Uracil-DNA glycosylase 1 & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: 2 µg/mL from a representative lot detected UNG1 in human heart tissue.
Immunofluorescence Analysis: 20 µg/mL from a representative lot detected UNG1 in human heart tissue.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Biochem/physiol Actions
This antibody recognizes the C-terminus of UNG1.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
C2C12 cell lysate
Evaluated by Western Blotting in C2C12 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected UNG1 in 15 µg of C2C12 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| UniProt accession no. | P13051 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UNG(7374) |
