General description
Anti-Giα-1-Subunit, Internal (159-168), rabbit polyclonal, recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. It is validated for use in Western blotting.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes Giα-1 subunit.
Recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits.
Immunogen
a synthetic peptide (LDRIAQPNYI) corresponding to amino acids 159-168 of mouse Giα-1, conjugated to KLH
Application
Immunoblotting (1:1000)
Physical form
Serum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, Goα and Gsα. Variables associated with assay conditions will dictate the proper working dilution.
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | diluted serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | mouse |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Immunoblotting (1:1000)) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −70°C |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Gnai1(14677) |
