General description
55-65 kDa observed
Transcription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences that encode for the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Sox9.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sox9 in HepG2 cells.
The unconjugated version (Cat. No. AB5535) is also shown to be suitable for Western blotting and immunohistochemistry applications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Sox9 Antibody, Alexa Fluor™ 488 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.
Physical form
Affinity purified
Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox9 in HeLa cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | chicken, rat, human, mouse |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), feline (based on 100% sequence homology), horse (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NP_000337 |
| UniProt accession no. | P48436 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SOX9(6662) |
