General description
E3 ubiquitin-protein ligase CBL-C (UniProt Q9ULV8; also known as RING finger protein 57, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C) is encoded by the CBLC (also known as CBL3, RNF57) gene (Gene ID 23624) in human. CBLC belongs to the CBL family of E3 ubiquitin ligases, members of which also include CBL (a.k.a. c-CBL) and CBL-B. Similar to CBL and CBL-B, CBL-C binds SH3 (Src homology 3) domain-containing proto-oncogenic tyrosine kinases such as EGFR, Lyn, Crk, Src and Ret. CBL-C is recruited to EGFR upon EGF stimulation, leading to CBL-C phosphorylation by EGFR and subsequent downstream MAP kinase signalling. In addition to mediating oncoprotein Ret ubiquitination for proteasomal degradation, CBL-C is shown to form a complex with HIC5 (Hydrogen peroxide-inducible clone 5) and the heat shock protein HSP27 that are necessary for the ubiquitination of NOX4. Unlike the other CBL family members, CBL-C expression appears to be restricted to epithelial tissues. Transgenic expression of CBL-C in mouse mammary gland causes impairment of cell proliferation, suggesting that CBL-C might have a growth-inhibitory role. CBL-C contains an N-terminal Cbl-type phosphotyrosine-binding (Cbl-PTB) domain (a.a. 7-321) that mediates SH3 domain interaction and a C-terminal RET-interacting region (a.a. 351-474) harboring the catalytic ring finger domain (a.a. 351-390) that mediates interaction with E2 ubiquitin-conjugating enzyme. The PTB domain, also known as tyrosine kinase-binding (TKB) domain, is subdivided into a four-helix bundle or 4H domain (a.a. 7-145), a calcium-binding EF hand-like domain (a.a. 146-218), and a SH2-like domain (a.a. 219-321).
~50 kDa observed. 52.46 kDa (isoform 1; Long) and 47.42 kDa (isoform 2; Short) calculated. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: RET-interacting region.
GST-tagged recombinant fragment from the RET-interacting region of human CBL-C.
Application
Research Category
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
This Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 is validated for use in Western Blotting for the detection of E3 ubiquitin-protein ligase CBL-C.
Biochem/physiol Actions
Clone 10F4.2 targets an epitope in the RET-interacting region present in both spliced isoforms of human CBL-C reported by UniProt (Q9ULV8).
Physical form
Format: Purified
Protein G purified.
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HT-29 human colon cancer cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected CBL-C in 10 µg of HT-29 human colon cancer cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 10F4.2, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001124324 |
| UniProt accession no. | Q9ULV8 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CBLC(23624) |
