General description
Thymosin beta-4 is the most prevalent of the cytoplasmic G-actin binding proteins belonging to the highly conserved thymosin beta family. Known to bind actin monomers, Thymosin beta-4 sequesters G-actin in a manner that prevents actin polymerization. Thymosin beta-4 plays several important roles including induction of tissue remodeling, wound healing in both cell and tissue injuries, and cell differentiation via mechanisms that have yet to be fully elucidated. It is involved in the regulation of the cytoskeleton, and thereby influences an array of cellular processes. Within the brain, Thymosin beta-4 is associated with the neurite extension and migration of neurons. Expression has been observed in various hemopoietic and lymphoid malignant cell lines.
~ 33kDa observed
Immunogen
GST-tagged recombinant protein corresponding to the internal domain of human Thymosin beta-4.
Application
Anti-Thymosin beta-4 Antibody is an antibody against Thymosin beta-4 for use in WB & IC.
Western Blot (Snap i.d.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Thymosin beta-4 in NIH/3T3, HeLa, and A431 cells.
Biochem/physiol Actions
Th antibody recognizes the internal domain of Thymosin beta-4.
Analysis Note
Evaluated by Western Blot in recombinant Thymosin beta-4.
Western Blot Analysis: 1 µg/mL of the antibody detected Thymosin beta-4 on 10 µg of recombinant Thymosin beta-4.
Control
Recombinant Thymosin beta-4
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_066932 |
| UniProt accession no. | P62328 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TMSB4X(7114) |
