General description
IKAROS, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. IKAROS has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.
~65 kDa observed. (Martin-Ibanez, R., et al. (2010). The Journal of Comparative Neurology. 518:329–351.) An uncharacterized band appears at ~57 kDa in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human IKAROS.
Application
Anti-IKAROS Antibody detects level of IKAROS & has been published & validated for use in WB & IC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected IKAROS in ReNcell
Research Category
Stem Cell Research
Research Sub Category
Transcription Factors
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IKAROS on 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat, human, mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_006051 |
| UniProt accession no. | Q13422 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... IKZF1(10320) mouse ... Ikzf1(22778) rat ... Ikzf1(305501) |
