General description
28 kDa calculated
Neutrophil Elastase, also known as Bone marrow serine protease, Elastase-2, Human leukocyte elastase (HLE), Medullasin or PMN elastase, and encoded by the gene ELANE/ELA2, is a serine proteinase in the same family as chymotrypsin. Neutrophil Elastase has broad substrate specificity and is part of our host defense mechanism against bacteria. Unfortunately passively released or actively secreted elastase from neutrophils has been linked to the pathologic processes of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome, and cystic fibrosis. Neutrophil Elastase is localized to the cytoplasm in granules. Neutrophil Elastase is expressed in neutrophils and macrophages. Defects in Neutrophil Elastase may be associated with cyclic neutropenia and congential neutropenia diseases where neutrophils fail to mature leaving the patient very vunderable to infections.
Immunogen
Whole cells corresponding to human Neutrophil Elastase.
Application
Detect Elastase using this mouse monoclonal antibody, Anti-Neutrophil Elastase Antibody, clone AHN-10 validated for use in ICC, IHC, IP, Radioimmunoassay & Inhibition.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in unirradiated and irradiated human and mouse skin tissues (Rhodes, L. E., et al. (2009). FASEB J. 23(11): 3947-3956.; Nicolaou, A., et al. (2012). Photochem Photobiol Sci. 11(2):371-380.; Rutter, J. J., et al. (2009). 129(12):2861-2867.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human acinar cells (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).
Inhibition Analysis: This assay inhibits the elastinolytic actiity of purified human Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.).
Radioimmunoassay Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase using purified Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.).
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Biochem/physiol Actions
This antibody detects with human Neutrophil Elastase but not other major neutrophil proteases: Cathapsin G, proteinase 3, collagenase, or esterase N (Skubitz, K. M., et al. (1988). J Leukoc Biol. 44(3):158-165.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry Analysis in peripheral blood mononuclear cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Neutrophil Elastase in peripheral blood mononuclear cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | AHN-10, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,inhibition assay: suitable,radioimmunoassay: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001963.1 |
| UniProt accession no. | P08246 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ELANE(1991) |
