General description
DPPA-2 (developmental pluripotency associated-2) is a novel Oct-4-related protein that is localized to the nucleus of mouse fetal germ cells. DPPA-2 has binding sites for both POU and Sox-2 protein domains and is expressed primarily in the developing germ line following gastrulation. These associations suggest that this protein participates in a very strong supportive role in maintaining cell pluripotentiality. In addition to its role as a pluripotency marker, more recent research using cancer cells suggests that DPPA-2 may also act as an embryo-cancer antigen—pointing to the presence of abnormal cell growth and the formation of cancerous cells.
~30 kDa observed
Immunogen
recombinant protein corresponding to mouse DPPA-2.
Application
Detect DPPA-2 using this Anti-DPPA-2 Antibody, clone 6C1.2, Cy3 conjugate validated for use in ICC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Physical form
Protein G Purified
Purified mouse monoclonal IgG2aλ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Mouse Embryonic Stem Cells (SCR012).
Evaluated by Immunocytochemistry in Mouse Embryonic Stem Cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected DPPA2 in Mouse Embryonic Stem Cells (SCR012).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | CY3 conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 6C1.2, monoclonal |
| species reactivity | mouse |
| technique(s) | immunocytochemistry: suitable |
| isotype | IgG2a |
| NCBI accession no. | NP_082891 |
| UniProt accession no. | Q9CWH0 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Dppa2(73703) |
