General description
BTG3 is a tumor suppression protein because overexpression impairs serum-induced cell cycle progression from G0/G1 to the S phase in vitro. BTG3 is often down regulated in cancer cell lines, and thus BTG3 is a target for enhanced cancer therapy. In normal cells BTG3 inhibits cell proliferation, cell invasion, and angiogenesis, and also regulates cell-cycle progression and differentiation in a variety of cell types. Developmentally BTG3 is normally expressed primarily in the brain and CNS during development where is modulates cell proliferation. In the adult expression is high in ovary, testis, prostate, thymus and lung. It is not expressed strongly in many cancers.
~37 kDa observed. Uncharacterized bands may be observed in some lysates. The calculated molecular weight of this protein is 29 kDa, but can be observed at ~37 kDa due to alternate splice variants.
Immunogen
GST-tagged recombinant protein corresponding to human BTG3.
Application
This Anti-BTG3 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of BTG3.
Research Sub Category
Apoptosis - Additional
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BTG3 in human testis and human prostate tissue.
Research Category
Apoptosis & Cancer
Biochem/physiol Actions
This antibodies immunogen is 94% identical in mouse and 92% identical in rat.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in IMR-90 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected BTG3 in 10 µg of IMR-90 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_006797 |
| UniProt accession no. | Q14201 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... BTG3(10950) |
