Application
Goat anti-Mouse IgG Antibody, Rhodamine conjugate detects level of Mouse IgG & has been published & validated for use in IF.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Suggested dilution for most applications: 1:50-1:200 Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Mouse IgG
WAVELENGTH:
Absorption peak = 550 nm, Emission peak = 570 nm.
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with 2 mL of sterile distilled water
RHODAMINE/PROTEIN:
Approximately 0.43 (A550/A280).
Preparation Note
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Segment | 100 |
| conjugate | TRITC conjugate,rhodamine conjugate |
| antibody form | F(ab′)2 fragment of affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunofluorescence: suitable |
| shipped in | wet ice |
| target post-translational modification | unmodified |
