General description
The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports sµggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
This rat monoclonal antibody Anti-Ly-6G (Gr-1) (mouse), PE-Cy7, clone RB6-8C5 validated for use in IHC, Flow Cytometry & IP for the detection of Ly-6G (Gr-1).
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected Ly-6G (Gr-1) in one million C57Bl/6 bone marrow cells.
| biological source | rat |
| Quality Level | 100 |
| conjugate | PE-Cy7 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | RB6-8C5, monoclonal |
| species reactivity | mouse |
| concentration | 0.2 mg/mL |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2bκ |
| UniProt accession no. | P35461 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Ly6G(546644) |
