General description
The 15.2 antibody reacts with human CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1), a 90-110 kDa cell surface glycoprotein that is inducibly expressed on both immune and endothelial cells. As its name implies, ICAM-1 participates in cell-cell adhesion between leukocytes and endothelial cells, facilitating leukocyte recruitment and transmigration at sites of inflammation. The ligands for ICAM-1 are also expressed on leukocyte and endothelial cells, and include Mac-1, fibrinogen, and a member of the integrin protein family, LFA-1 (CD11a).The 15.2 antibody may be used for analysis of ICAM-1 expression in human cells and tissues, and is reported to be cross-reactive with porcine ICAM-1.
This mouse monoclonal antibody Anti-CD54 (ICAM-1) (human), PE, clone 15.2 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD54 (ICAM-1).
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD54 (ICAM-1) in one million human peripheral blood monocytes.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | PE |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | 15.2, monoclonal |
| species reactivity | human |
| packaging | pkg of 5 μL (0.5 μg/test) |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P05362 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ICAM1(3383) |
