General description
Human BAFF (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family. BAFF plays an important role as co-stimulator of B-cell proliferation and function. Recombinant human BAFF is a soluble 17.0 kDa protein containing 153 amino acid residues, comprising the TNF-like extracellular domain of BAFF.
Product Source: E.coli
Physical form
Lyophilized from sterile filtered 0.5X PBS, pH 7.5, and 0.5 mM DTT, containing no additives.
Preparation Note
The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. Reconstituted material can be stored at 2°-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles.
Analysis Note
Specific Activity: Human BAFF is biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on human RPMI 8226 cells is 5-10 ng/mL.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| assay | >98% (SDS-PAGE) |
| mol wt | Mw 17.0 kDa |
| manufacturer/tradename | Chemicon® |
| technique(s) | cell culture | stem cell: suitable |
| impurities | <0.1 ng/μg Endotoxin (of BAFF; 1 EU/ug) |
| input | sample type induced pluripotent stem cell(s) sample type: human embryonic stem cell(s) |
| NCBI accession no. | NM_006573.3 |
| UniProt accession no. | Q9Y275 |
| shipped in | dry ice |
| storage temp. | -10 to -25°C |
