General description
A colorimetric assay kit for the measurement of the total antioxidant status. Each kit is suitable for performing a minimum of 50 assays.
Note: Spectrophotometer equipped with a temperature-controlled cuvette holder is strongly recommended for use with this kit.
Biochem/physiol Actions
The Calbiochem
Preparation Note
• Chromogen: Add 10 ml Buffer to each vial of Chromogen needed for the assay (10 tests per vial). After reconstitution, the Chromogen is stable for 2 days at 4°C or 8 h at room temperature.
• Substrate: Dilute each vial of Substrate needed for the assay by adding 7.5 ml Buffer. After dilution, the substrate is stable for 24 h at 4°C. NOTE: Some automated methods do not require substrate dilution.
• Standard: Add 1 ml distilled water to each vial of Standard needed for the assay. After reconstitution, the Standard is stable for 2 days at 4°C or 1 month at -20°C.
Upon arrival store the entire kit contents at 4°C. Note: The concentration of the standard is lot-specific. Please refer to the vial label for lot-specific concentration.
Analysis Note
Calculation of Total Antioxidant StatusThe antioxidant concentration of the Standard is used to calculate the antioxidant levels in the samples.
1. Determine the ΔA for the samples, standard, and blank. ΔA = A - Ao
2. Calculate the concentration of antioxidants in the sample using the formula below:
NOTE: If the antioxidant concentration is greater than 2.5 mM dilute the sample with 0.9% NaCl and re-assay. Typical antioxidant levels in normal human plasma are 1.30-1.77 mM.
Other Notes
Miller, N.J., et al. 1993. Clin. Science 84, 407.
Substrate, Chromogen, Standard, Buffer, and a user protocol.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| usage | sufficient for 50 tests |
| Quality Segment | 100 |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| assay range | standard curve range: 0-2.5 mM (plasma) |
| input | sample type serum sample type plasma sample type tissue extract(s) sample type beverage(s) sample type plant extract(s) |
| detection method | colorimetric |
| shipped in | ambient |
| storage temp. | 2-8°C |
