General description
Interleukin-10 (IL-10) is an anti-inflammatory cytokine primarily produced by regulatory T cells, macrophages, and dendritic cells, playing a crucial role in modulating immune responses. It is essential for inhibiting the production of pro-inflammatory cytokines, thus helping to maintain immune homeostasis and prevent excessive inflammation. IL-10′s regulatory functions make it a key player in various diseases, including autoimmune disorders and chronic inflammatory conditions, highlighting its potential as a therapeutic target.
Application
Inflammatory Disease / Immune Response / Autoimmune Disease
Features and Benefits
- Femtogram-Level Sensitivity for IL-10 Detection: Enables quantification of extremely low levels of IL-10, critical for studying subtle anti-inflammatory responses in immunology and autoimmune disease research.
- Elution-Based Workflow for Low Background: Reduces background noise and improves signal-to-noise ratio, enhancing assay performance in complex biological samples.
- Comprehensive Kit for Streamlined Assay Setup: Offers a ready-to-use solution for researchers, minimizing setup time and ensuring consistency across experiments.
- Supports Autoimmune and Inflammatory Disease Research: Enables quantification of IL-10 in conditions like rheumatoid arthritis, lupus, and psoriasis, where IL-10 plays a dual role in inflammation and immune tolerance.
Packaging
96 well assay
Analysis Note
- Sensitivity: LLOQ 100 fg/mL* (SMCxPRO® Instrument)
- Standard Curve Range: 100-100,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 106% Plasma: 106%
- Recovery: Serum: 87% Plasma 90%
Other Notes
Although IL-10 was detectable in non-human primate, mouse, rat, canine and feline samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| description | Used for ultrasensitive quantification of Human IL-10 in human serum and plasma |
| product line | SMC® |
| usage | kit sufficient for 96 wells |
| species reactivity | human |
| technique(s) | single molecule counting: suitable |
| input | serum |
| accession no. | P22301 |
| compatibility | for use with FemtoQuest™ and SMCxPRO® instruments |
| shipped in | wet ice |
| storage temp. | 2-8°C |
