General description
BIOCHEMISTRY: pI = 8.0
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.
Immunogen
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.
Application
Anti-HLA-B27 Antibody, clone HLA-ABC-m3, FITC conjugated detects level of HLA-B27 & has been published & validated for use in FC & IF.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.
SUGGESTED USAGE DILUTION
Flow cytometry and immunofluorescence 1:15 to 1:60
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Biochem/physiol Actions
This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7
(9.5x107M-1).
Physical form
Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in 0.5ml phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.
Preparation Note
Store at 2 to 8°C. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | FITC conjugate |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | HLA-ABC-m3, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunofluorescence: suitable |
| isotype | IgG2a |
| NCBI accession no. | NM_005514.5 |
| UniProt accession no. | P01889 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HLA-B(3106) |
