General description
Selenoprotein K (SELK) is a selenoprotein that is localized at the endoplasmic reticulum, and highly expressed in the heart. SELK functions as an antioxidant that can bind to proteins such as the ER-associated degradation (ERAD) components p97 ATPase, Derlin-1 and -2, and SelS as well as proteins in the oligosaccharyltransferase complex. SELK may be involved in the Derlin-dependent ERAD of glycosylated misfolded proteins, is thought to be important in Ca2+ flux in immune cells, and is cleaved by m-calpain in resting macrophages.
~10 kDa observed. Uncharacterized bands may appear in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to human SELK.
Application
Anti-SELK Antibody is a highly specific rabbit polyclonal antibody, that targets Selenoprotein K & has been tested in western blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A20 cell lysate
Evaluated by Western Blotting in A20 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SELK in 15 µg of A20 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human (immunogen homology) |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9Y6D0 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SELK(58515) |
